Fig. 5
In vivo experiments revealed the role of CYFIP2 in ESCC. (A,B) RT-qPCR and western blot were used to verify the successful establishment of stably transfected cells. (C) The growth curve of subcutaneous xenograft tumor in mice during the experiment (Unit: mm3; shCtrl + PBS vs shCYFIP2 + PBS: 1264.509 ± 176.871 vs 985.390 ± 263.207, p-value = 0.0247; shCtrl + CDDP vs shCYFIP2 + CDDP: 417.525 ± 194.566 vs 206.827 ± 49.873, p-value = 0.0464; n = 5). (D) Photos of xenograft tumor and size post-euthanasia of mice. (E) Measurement and analysis of xenograft tumor weight (Unit: g; shCtrl + PBS vs shCYFIP2 + PBS: 1.13 ± 0.435 vs 0.484 ± 0.190, p-value = 0.0160; shCtrl + CDDP vs shCYFIP2 + CDDP: 0.81 ± 0.4078 vs 0.322 ± 0.182, p-value = 0.0403; n = 5). (F) The tumor tissues were prepared by paraffin wax and stained by HE and IHC (CYFIP2 and LC3I/II). (G) The paraffin sections were stained by TUNEL.
