Fig. 4 | Scientific Reports

Fig. 4

From: Transcriptome analysis to identify genes related to programmed cell death resulted from manipulating of BnaFAH ortholog by CRISPR/Cas9 in Brassica napus

Fig. 4

Gene expression of westar, bnaa06fah and bnafah mutant under 0 (LD), 1(SD1) and 2 (SD2) short day. (A) Experimental design with each sample hybridized against a reference RNA (solid arrows) enables multidirectional comparisons between genotypes and treatments with a linear mixed model (dotted arrows). The experiment described was repeated at each time point (0, 1, 2 short days) and lists of differentially expressed transcripts (log2 ratio ± 1, q < 0.05) were made from each comparison. (B) Venn diagram showing the overlap of gene lists. Transcripts at least two-fold differentially regulated in comparisons between group1-4 (group1 and group2 is the DEGs expressed in bnafah compared with Westar (wild-type) under SD1 and SD2; group3 and group 4 is the DEGs expressed in bnafah compared with bnaa06fah under SD1 and SD2). (C) Statistical analysis of DEGs in different groups in different periods. The Number of genes with higher (westar > bnaa06fah; westar > bnafah; bnaa06fah > bnafah) and lower (westar < bnaa06fah; westar < bnafah; bnaa06fah < bnafah) expression under short-day for 0 (LD), 1 (SD1) and 2 (SD2) days was calculated. (D) Heatmap of BnaA06FAH and BnaCO5FAH expression in wild-type (westar), bnaa06fah and bnafah under short day for 0 (LD), 1 (SD1) and 2 (SD2) days.

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