Fig. 3
Effects of IL-10 on the production of IL-6, but not on TNF, by BMCMCs depend on STAT1 and STAT3. BMCMCs (A) or 0.5 µM cucurbitacin I-treated BMCMCs (B,C) (n = 5–10 derived from distinct mice) were stimulated with or without IgE (SPE-7) in the presence or absence of 1–100 ng/mL IL-10 for 24 h. The levels of IL-6 and TNF in the culture supernatants were determined using ELISA. (A) The cultivation using Stat1+/+ and Stat1−/− BMCMCs. *p < 0.05, Stat1+/+ BMCMCs vs. Stat1−/− BMCMCs. (B) Culture of cucurbitacin-I-treated wild-type BMCMCs. *p < 0.05, vehicle-treated BMCMCs vs. cucurbitacin I-treated BMCMCs. (C) The cultivation using cucurbitacin I-treated Stat1−/− BMCMCs. Data show the mean ± SEM and the representative result of two independent experiments.
