Fig. 4
IL-10 promotes Th17 cell differentiation by enhancing STAT1-dependent IL-6 production in IgE-stimulated mast cells. Naïve CD4+ T cells were co-cultured with wild-type and Il6−/− BMCMCs (n = 4–5 derived from distinct mice) or with Stat1+/+ and Stat1−/− BMCMCs (n = 4–5 derived from distinct mice) in the presence of anti-mouse CD3 mAb and IgE (SPE-7), with or without 100 ng/mL rmIL-10, for 4 days. The cells were then stimulated with PMA + ionomycin in the presence of monensin for 6 h. Intracellular IL-17 and Foxp3 expression levels were determined by FACS analysis. (A) Representative FACS results. (B) Data show the mean ± SEM from (A), and the representative results of two independent experiments. *p < 0.05, medium vs. rmIL-10, and †p < 0.05, wild-type BMCMCs vs. Il6−/− BMCMCs.
