Fig. 2

Isolation and identification of DPSCs. (A) DPSCs crawling out of the pulp tissue mass were pike-shaped. (B) Results of alizarin red staining with visible calcium nodules. (C) The result of ALP staining shows that the cells are bluish-purple. (D) Oil red O staining results in visible orange lipid droplets (The lipid droplets are indicated by red arrows) and nuclei stained blue by hematoxylin. (E) The phenotype of DPSCs was analyzed by flow cytometry.