Table 1 Western blot conditions and list of antibodies used in the present study

From: Effect of carbon black and silicon dioxide nanoparticle exposure on corona receptor ACE2 and TMPRSS2 expression in the ocular surface

Antigen

Source

Primary antibody dilution

Secondary antibody dilution

ACE2

Abcam (catalog no ab108252)

1:1000

1:2000

AngII

novusbio (catalog no NB100-62346)

1:1000

1:2000

TMPRSS2

Abcam (catalog no ab214461)

1:1000

1:2000

Anti-mouse antibody

Cell Signaling Technology (catalog no. 7076S)

 

1:2000

Anti-rabbit antibody

Cell Signaling Technology (catalog no. 7074S)

 

1:2000