Fig. 7

Summary of dimension reduction and classification of biochemical and molecular responses in L. album cells exposed to MeJA at the 6, 12, 24, 48, and 72 h time points. (a) Partial Least Squares Discriminate Analysis (PLS-DA) scores plot is used to classify data obtained from the untreated and treated cells. MeJA treatment are shown as M6, M12, M24, M48, and M72. Controls are shown with signs C6, C12, C24, C48, and C72. (b) Hierarchical Cluster Analysis (HCA) is applied to cluster data based on Pearson correlations coefficient. Data are shown for three replications for each variable during MeJA treatment. Colours in the HCA map show the magnitude and direction of correlations. The blue box means a strong negative correlation, and the red box signifies a strong positive correlation. The primary clusters are numbered 1 to 5 in the picture. (c) A summary of this research in which SA molecule can play a crucial role in MeJA responses in L. album cells. An increase in SA can affect H₂O₂ as a crucial second messenger for inducing phenylpropanoid pathway through changes in the expression levels of miR156, miR159, miR172, miR396, and CCR.