Table 2 Sequences of primers used for RT-PCR.

From: Human platelet lysate combined with mesenchymal stem cells pretreated with platelet lysate improved cardiac function in rats with myocardial infarction

Genes

Primer sequence 5’—3’

Reference

miR-1-3p

F: GGCTGGAATGTAAAGAAGTG

60

R: TATCCAGTGCGTGTCGTG

RT: GTCGTATCCAGTGCGTGTCGTGGAGTCGGCAATTGCACTGGATACGACATACACAC

miR-133a-3p

F: GCGTTTGGTCCCCTTCAAC

61

R: AGTGCAGGGTCCGAGGTATT

RT: GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACCAGCTG

miR-499a-3p

F: GGGAACATCACAGCAAGTC

61

R: CAGTGCGTGTCGTGGAG

RT: GTCGTATCCAGTGCGTGTCGTGGAGTCGGCAATTGCACTGGATACGACAGCACA

miR-208a-3p

F: ACACTCCAGCTGGGATAAGACGAGCA

62

R: TGGTGTCGTGGAGTCG

RT: CTCAACTGGTGTCGTGGAGTCGGCAATTCAGTTGAGGCTTTTTG

GATA4

F: ACCCATCACACAGATCGCAG

63

R: TGTTCAGGCTGGAGAGCAAG

Nkx2.5

F: CCTCGGATTTCACACCCACA

64

R: CCGAGGCATCAGGTTAGGTC

Oct4

F: CATCTGCCGCTTCGAG

65

R: CTCAATGCTAGTCCGCTTTC

Nanog

F: GCCCTGATTCTTCTAGCAAT

65

R: AGAACACAGTCCGCATCTT

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