Fig. 4
From: Optogenetic activation of serotonergic neurons changes masticatory movement in freely moving mice
Optogenetic activation of DRN 5-HT neurons does not induce masticatory movement initiation but modulates ongoing mastication movement. (a) Representative EMG recordings of digastric and masseter muscles before and after blue light application (blue bar) during resting state. (b) Pooled data for the percentage of EMG activity evoked after yellow (control) or blue light (activation) illumination to that during the resting state before yellow or blue light illumination in the digastric and masseter muscles. (c) Representative EMG recording of digastric and masseter muscles before and after blue light application (blue bar) during incision (left). The dashed line indicates the start of feeding. Expanding EMG recordings in dotted box outlines of left EMG recordings (right). (d) Representative EMG recording of digastric and masseter muscles before and after blue light application (blue bar) during chewing stage (left). Expanding EMG recordings in dotted box outlines of left EMG recordings (right). (e) Pooled data for masticatory frequency after blue light application during incision and chewing stages. (f) Pooled data for periods showing high frequency (> 7 Hz) masticatory muscle activity after blue light application during incision and chewing. (g) and (h) Pooled data for RMS peak amplitude (f) and duration (g) in masseter EMG bursts after blue light application during incision and chewing. (i) and (j) Pooled data for RMS peak amplitude (h) and duration (i) in digastric EMG bursts after blue light application during incision and chewing. **P < 0.01, ***P < 0.001 (unpaired Student’s t test). Comparisons with n.s. have P > 0.05 and are considered not significant. Data are expressed as mean ± SEM.
