Table 1 The list of selected candidate epitopes with their properties for designing the final vaccine construct.

From: Bioinformatics analysis to design a multi-epitope mRNA vaccine against S. agalactiae exploiting pathogenic proteins

Cell Type

Protein name

Sequence of Epitope

Percentile Rank

IC50 Value

Predicted MHC Binding

Affinity

Rescale Binding

Affinity

C-terminal Cleavage

Affinity

Transport

Affinity

Prediction

Score

MHC

Ligand

MHC I binding Alleles

CD8 + Cytotoxic T Lymphocytes

Sip

NIADINLIY

0.93

305.75

0.5684

2.4133

0.8963

3.2480

2.7102

✓

HLA-B*35:01

Sip

NMAANNISY

0.12

3.80

0.4459

1.8934

0.8881

3.0510

2.1791

✓

HLA-B*15:01

Sip

YKEKVASTY

0.6

212.27

0.2870

1.2185

0.9655

2.9640

1.5115

✓

HLA-A*30:02

Rib

NSPDLKAQY

0.28

77.25

0.2155

0.9151

0.9674

3.0350

1.2119

✓

HLA-A*26:01

Rib**

TVGAASINY

0.71

251.26

0.1803

0.7657

0.8460

3.0630

1.0457

✓

HLA-B*15:01

Cpa-g**

STGRASLTY

0.49

106.11

0.6135

2.6047

0.9703

2.7440

2.8874

✓

HLA-A*01:01

Cpa-g

IVSVALTIY

0.42

80.12

0.2371

1.0069

0.9658

2.9430

1.2989

✓

HLA-A*26:01

 

Cpa-g

QTDIDSKIV

0.54

140.62

0.2409

1.0230

0.6644

0.1400

1.1296

✓

HLA-A*01:01

CD4 + helper T Lymphocytes

Protein name

Sequence of Epitope

Percentile Rank

Core of Log50k (aff)

Level of Binding

Alleles

Sip

ISEAMSIDMNVLAKI

0.78

0.7445

Strong binder

HLA-DRB1_0301

Sip

KYGDTLSVISEAMSI

0.58

0.5312

Strong binder

HLA-DQA10201-DQB10202

Sip

PMKTYSSAPALKSKE

0.84

0.7718

Strong binder

HLA-DRB1_0401

Rib

IKKFKFGAASVLIGI

0.91

0.8626

Strong binder

HLA-DRB1_0101

Rib

TVFAAEVISGSAVTL

0.97

0.8819

Strong binder

HLA-DQA10201-DQB10301

Rib

PSELTTVGAASINYT

0.98

0.8893

Strong binder

HLA-DQA10201-DQB10301

Cpa-g

KKFKFGAASVLIGLS

0.86

0.8621

Strong binder

HLA-DRB1_0101

Cpa-g

LIVLEQGFTAKYVFR

0.69

0.7426

Strong binder

HLA-DRB1_0101

B Lymphocyte

Protein name

Sequence

Start position

End position

Score

Sip

SMKIETPATNAAGQTT

106

121

0.97

Sip

TYRAGDPGDHGKGLAV

346

361

0.97

Sip

ASVAAETPAPVAKVAP

224

239

0.93

Rib

TVKVVDPRTDADKNDP

1011

1026

0.93

Cpa-g

TVHVTPKPVPDKDKYD

957

972

0.94

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