Fig. 3

Interaction between both species in mixed biofilm: (a) Schematic overview of the methodology developed to evaluate the interaction between bacterial species: i.S. aureus Xen36 single biofilm culture without insert. ii.S. aureus Xen36 and P. aeruginosa PAO1 mixed biofilm without insert. iii.S. aureus Xen36 and P. aeruginosa single biofilm in the insert. iv.S. aureus Xen36 single biofilm, and S. aureus Xen36 and P. aeruginosa PAO1 mixed biofilm in the insert. (b) Bioluminescence signal of S. aureus Xen36 over 48 h. (c)S. aureus Xen36 counting measured after 48 h.