Fig. 2 | Scientific Reports

Fig. 2

From: Exploring the Endothelin-1 pathway in chronic thromboembolic pulmonary hypertension microvasculopathy

Fig. 2

Human Pulmonary Endothelial Cells (PMECs) Exhibit Increased ET-1 Expression in Regions of Disturbed Flow. (A) A schematic representation of the experimental setup for cell culture under flow within a Y-shaped ibidi plate. (B) The diagram indicates the expected shear stress distribution and highlights the image locations (i-v) for subsequent analyses. This schematic was generated using Biorender. (C) Immunofluorescent staining of PMECs at specified regions within the plate, showing ET-1 expression (red), VE-cadherin (green) and DAPI (blue). The scale bar in all staining images is set at 100 µm.

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