Fig. 6 | Scientific Reports

Fig. 6

From: pH-Dependent migratory behaviors of neutrophil-like cells in a microfluidic device with controllability of dissolved gas concentrations

Fig. 6

Measurement of migration of neutrophil-like HL-60 cells under a pH gradient with chemical stimuli by using the microfluidic device placed in a stage incubator controlled at 5% CO2. Temporal and spatial variations in average migration speed of the cells under the pH gradient with supplementation of (a) 10 nM fMLP, (b) 1 mM pyruvate sodium, or (c) 10 µM cariporide (mean ± S.D., n = 3), and (d) variation in the migration speed with pH between 2 and 3 h after the experiment under the pH gradient with/without the chemical stimuli.

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