Table 4 Primers used in this study: the nucleotide positions are based on RHDV strain Genbank accession X87607.
Name | Sequence | Length (bp) | Position nt Genome (~ 7437 bp) | |
|---|---|---|---|---|
VP60 | ||||
5310-F | 5′-CCTGACGACGAATTTGTGAA-3′ | 913 | 5281 | 5300 |
RCV-1593Rev | 5′-TGGTCTATGTCGGTAAACCG-3′ | 6194 | 6175 | |
RCV750-F | 5′-CCAGTCCTTACTGGGGTTGGC-3′ | 992 | 6040 | 6060 |
RCV07-7004R | 5′-TCAGACATAGGAAAAGCCATTGG-3′ | 7032 | 7012 | |
VP607-F | 5′-ACYTGACTGAACTYATTGACG-3′ | 104 | 6941 | 6961 |
VP608-R | 5′-TCAGACATAAGAAAAGCCATTGG-3′ | 7044 | 7022 | |
RCV-1731R | 5′-TCAGACATAGGAAAAGCCACTGGTT-3′ | 1391 | 6959 | 6983 |
RCV-301F | 5′-CCACAAAACAACCCGTTCACAACT-3′ | 5593 | 5613 | |
RCVCOOH-R | 5′-CAGTTCYTGCCTGCGGAGCA-3′ | 1062 | 7102 | 7083 |
RCV750ANCI-F | 5′-CCAGTTCTCACTGGGGTCGGC-3′ | 6040 | 6056 | |
RCV270-F | 5′-GTACACTGCCCAGCAYTCACCA-3′ | 722 | 5522 | 5544 |
RCV992-R | 5′-GTCGGGAAAACCATCCGGGGCA-3′ | 6222 | 6244 | |
NPS | ||||
RCV_R2_902_R | 5′-GTCCAGTCCATGAGTATTGC-3 | 902 | 902 | 883 |
24-F | 5′-GTGAAAGTTATGGCGGCTATGTCG-3′ | 1 | 24 | |
RCV-843-F | 5′-TGCTGGTTGGCCTCCACAATGTCA-3′ | 830 | 842 | 865 |
RCV-1650-R | 5′-CGAAGTGGTCAAGGCCAAAGTTGA-3′ | 1649 | 1672 | |
RCV-R2_1525_F | 5′-GTCATTTT(Y)AAAGG(R)GCCCCAG-3′ | 719 | 1528 | 1546 |
RCV_R2_2225_R | 5′-TACTGTTTCCAACAT(R)AG(M)AG-3′ | 2226 | 2247 | |
RCV_R2_2123_F | 5′-AGGGTGCCAACAAGTTCAACTT-3′ | 927 | 2123 | 2144 |
RCV_R2_3062_R | 5′-TCTCTACG(Y)GCAGCTTGCCA-3′ | 3050 | 3031 | |
RCV_R2_3650_R | 5′-GTGTGTATTGCGACAATCTT-3′ | 698 | 3674 | 3655 |
RCV-3000_F | 5′-ACAAAGGCTTTCCAGGGTGTGAAAG-3′ | 2976 | 3000 | |
RCV-5375-R | 5′-GGGGACTGATGCTGTGGTAG-3′ | 1781 | 5345 | 5364 |
RCV-3585-F | 5′-GACTCTACCAAGATTGACGTGCT-3′ | 3583 | 3605 | |
RCV-3922-F | 5′-GACCCAGACAGTGACAAGTCCCT-3′ | 618 | 3922 | 3944 |
RCV-4540-R | 5′-CAACACATGGGGACATAGTGGAGT-3′ | 4540 | 4517 | |
