Fig. 7

Physical interaction may be required for production of 1. (a) Micrographs obtained from single or co-culture after 2 days; right; illustration of image of co-culture. (b) dialysis experiments (c) addition of cell culture supernatant (d) disruption of immune cell structure by sonication. Co-culture was performed for (b) 1 week and (c), (d) 2 weeks, respectively (see “Methods” for detail experimental methods and cultural conditions).