Fig. 1

Phylogenetic analysis of orthopoxvirus (OPV). (A) Enlarged unrooted phylogenetic tree illustrating genetic distances within the OPV family. The UPGMA algorithm was used for phylogenetic tree construction. Old-world OPVs define species such as monkeypox (Mpox), vaccinia virus (VACV), and variola virus as monophyletic clades. (B) Global phylogeny of Mpox includes the current predominant 2022 Mpox outbreak variant, strain Zaire-96-I-16 (NC_003310.1), and Mpox virus strain OP382478.1. The red box highlights significant similarities between previously identified Mpox virus proteins and recently discovered counterparts.