Fig. 4

Drug repurposing discovery in Mpox infection. This figure illustrates the results of the Steiner tree analysis used to uncover molecular pathways connecting DEGs in Mpox infection, focusing on (A) kinases and (B) transcription factors. The analysis identifies critical nodes and edges within the kinase and transcription factors networks, highlighting the key signaling pathways disrupted by Mpox. The screening of repurposed drugs was conducted based on these identified biological processes and signaling pathways significantly impacted by the infection. This analysis provides insights into potential therapeutic strategies for managing Mpox infection through the repurposing of existing drugs. Proviral genes associated with Mpox infection are represented by red ovals, while antiviral genes are shown in green ovals. FDA-approved drugs targeting proviral genes are indicated by green diamonds, and those targeting antiviral genes are shown in red diamonds. Additionally, yellow diamonds represent drugs that target both antiviral and proviral pathways.