Fig. 3

LKB1 in peripheral sensory neurons promotes ovarian innervation. (a) Graphic schema of the experimental methods used for retrograde tracing of ovarian innervation. (b) The number of fluorogold-positive neurons per DRG. (WT: n = 5 mice; LKB1^fl/fl: n = 4 mice, Na_v1.8^LKB1: n = 4 mice.) For each group, we obtained n = 5 technical replicates per DRG. (c) The number of Na_v1.8-positive neurons with positive fluorogold signal per DRG. (WT: n = 5 mice; LKB1^fl/fl: n = 4 mice, Na_v1.8^LKB1: n = 4 mice.) For each group, we obtained n = 5 technical replicates per DRG. (d) The number of TH-positive neurons with positive fluorogold signal per DRG. (WT: n = 5 mice; LKB1^fl/fl: n = 4 mice, Na_v1.8^LKB1: n = 4 mice.) For each group, we used n = 5 technical replicates per DRG. (e-f) Representative confocal microscopy (30X) of fluorogold (red), Na_v1.8 (green), and TH (purple) immunohistochemistry in the T12 DRG from LKB1^fl/fl (e) and Na_v1.8^LKB1 (f) females. Scale bar = 50 μm. Arrows indicate positive fluorogold signal. *p < 0.05, **p < 0.01, ***p < 0.0001, ****p < 0.0001 by repeated measures two-way ANOVA (d–f).