Fig. 4
From: Sensory neuron LKB1 mediates ovarian and reproductive function
Histological analysis of ovary morphology in Nav1.8LKB1females. (a) Graphic depiction of assessment of the follicular life cycle. (b) Ovary size as measured immediately following extraction (LKB1fl/fl: mean ± SEM = 2.6 ± 0.12 mm, n = 8 females; Nav1.8LKB1: mean ± SEM = 3.2 ± 0.27 mm, n = 6 females). (c) Number of corpora lutea (CL) per ovary (LKB1fl/fl: mean ± SEM = 2.0 ± 0.45 CL, n = 6 females; Nav1.8LKB1: mean ± SEM = 2.1 ± 0.55 CL, n = 8 females). (d) Representative images of H&E-stained ovary sections from females in estrus. Scale bar = 100 μm. *= CL. (e) Number of follicles at each stage. (LKB1fl/fln = 12 females; Nav1.8LKB1n = 8 females) For each group, n = 5–10 technical replicates/sections. (f) Representative images of H&E-stained perigonadal adipocytes. Scale bar = 20 μm. (g) Size (area in µm2) of perigonadal adipocytes. (LKB1fl/fl: mean ± SEM = 1531 ± 121.8 µm2, n = 14 females; Nav1.8LKB1: mean ± SEM = 1943 ± 104.2 µm2, n = 15 females). For each group, n = 5–10 technical replicates (sections) with a minimum of 60 cells analyzed per section. *p < 0.05, ***p < 0.0001 by unpaired two-tailed t-test (b, c, g) or ordinary two-way ANOVA (e). Open symbols indicate females in proestrus or estrus; closed symbols indicate females in metestrus or diestrus (b–d).
