Fig. 2

Microscopic images of colony expansion of radiation-exposed cells after PHA treatment. Microscopic images of T cell colonies following 12- and 24-hour culture of platelet product-derived MNCs in the presence of PHA 1% (a mitogen that specifically induces T cell expansion) were captured. Image (A) displays T cell colonies after 12-hour culture, whereas image (B) shows the colonies after 24-hours. Graphs (C) and (D) illustrate the significantly (P < 0.001) inhibitory effects of both gamma- and X-irradiations on T cell expansion and colony formation during both 12-hour and 24-hour culture, respectively. n = 5; ***, P < 0.001; ns, non-significant; MNC, mononuclear cell; PHA, phytohemagglutinin.