Fig. 7
Dysfunction of antioxidant and mitochondrial function in the unilateral anterior large overjet (UALO) group at 12 weeks post-bonding. (A) Downregulation of genes associated with the antioxidant system and mitochondrial function in RNA sequencing samples collected at 12 weeks post-bonding (n = 2 per sample). Slc25a4: Ant1; Ppargc1a: PGC-1α; Map1lc3a: LC3a. (B-C) Representative bands and quantifications of protein expression for SOD2, Pink1, Prkn, p62, and LC3 at 12 weeks post-bonding. n = 3 per group. *P < 0.05, **P < 0.01. (D) Representative images of the SOD2, Pink1, Prkn, p62, and LC3 immunohistochemical staining sections from tissues collected at 12 weeks post-bonding. Scale bar: 50 μm (upper panels), 20 μm (lower panels). (E) Quantifications of the average optical density (AOD) of the areas positive for SOD2, Pink1, Prkn, p62, and LC3 in 12 weeks post-bonding sections. AOD = IOD/area, n = 5 per group, **P < 0.01, ***P < 0.001.
