Fig. 7

Competitive PPIX binding. UV–visible absorbance spectra of 5 μM HSA-PPIX, HusA-PPIX, or HmuY-PPIX complexes were recorded. Then, samples were mixed with 5 μM apo-proteins, and the PPIX sequestration was analyzed by changes in the spectra recorded in time. HSA, human serum albumin.