Fig. 4
The paracrine of ESM1 on HUVECs in KIRC cell conditional medium. (A) workflow of conditional medium collection and analyses for further experiments. (B) The tube formation assay was performed in HUVECs cultured in conditional medium of KIRC cell transfected with ESM1 knockdown or empty vector (The number of biological replicates is n = 3, The magnification was × 100). The effect of paracrine of ESM1 on the proliferation ability of HUVECs by MTT assay (C) and EdU assay (D) (The number of biological replicates is n = 3). The migration ability of HUVECs cultured in conditional medium of KIRC cell transfected with ESM1 knockdown or empty vector was tested by transwell migration assay (E) (The number of biological replicates is n = 3, The magnification was × 100). *p < 0.05; **p < 0.01; ***p < 0.001. G1:
