Fig. 3

Structural analysis of decellularized vaginal wall matrix after chemical decellularization (DC) only, sterilization with 0.1%, 0.05% or 0.01% PAA/H₂O₂ treatment and AAE-based sterilization. Histological staining with Haematoxylin and Eosin (H&E) demonstrates damage to the ECM structure (red arrowheads) for sterilization with 0.1%, 0.05% or 0.01% PAA/H₂O₂ for 2 h sterilization at 125 rotations/min or 250 rotations/min constant agitation and for 3 h sterilization at 125 rotations/min, compared to a decellularized vaginal wall control sample without exposure to a sterilization procedure. Scalebars are 100–200 μm in length. Depicted histological images are a representation of triplicates from 3 donors (n = 9 per test condition). Scalebars are 100 μm in size.