Fig. 7

Evaluation of activity levels of mice on HFD treated with Veh or R159. (a, b) Voluntary wheel running exercise activity and behavior were measured before and during the drug treatment to assess activity levels. Multiple unpaired t-tests with FDR = 5% were used for the statistical analysis, q-values less than 0.05 were considered significant. (c) Motivation for voluntary exercise behavior was evaluated by counting the number of attempts. Multiple Mann-Whitney tests with FDR = 5% were used for the statistical analysis, q-values less than 0.05 were considered significant. (d) Hind leg muscle gene expression of Il-6, Ccl2, Tnfα, Il-1β, and Nos2 relative to housekeeping gene Rpl7 in mice on HFD treated by Veh or R159. Two-tailed Student’s t-test was used for statistical analysis, p-values less than 0.05 were considered significant.