Fig. 1 | Scientific Reports

Fig. 1

From: Salivary extracellular vesicles isolation methods impact the robustness of downstream biomarkers detection

Fig. 1

Workflow of human salivary extracellular vesicles isolation. Representative scheme of the workflow. Briefly, 4 mL of saliva were collected from donors and centrifuged successively at 300×g and 3000×g to remove cells and large debris and then small debris respectively. Then, whole saliva supernatant was splited in four fractions: 0.5 mL whole saliva supernatant (WS) used as a control and 1 mL dedicated to each EV isolation methods.

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