Table 1 Comparison of the three techniques used in this study for EV isolation.

From: Salivary extracellular vesicles isolation methods impact the robustness of downstream biomarkers detection

 

Ultracentrifugation

Coprecipitation

Immuno-capture

Mechanism of separation

Size and density

Centrifugation at 100,000 xG

Surface charge. Use of a cationic polymer to coprecipitate EVs

Specific binding of antibodies against EV markers tetraspanins CD9, CD63 and CD81 coated on magnetic beads

Specificity

Intermediate

Low

High

Time

High

Low

Intermediate

Cost

Intermediate

Low

High

Scalability

Low

High

Low

  1. In this study, we compared three isolation methods using a 3-level scale: high, intermediate, or low. The criteria for comparison were as follows: Specificity: The ability to effectively separate extracellular vesicles (EVs) from contaminants present in saliva. Time: The duration required to complete the EV isolation process, measured from the post-collection stage. Cost: The financial resources required to perform the isolation procedure. Scalability: The ability to process a large number of samples without significantly increasing time, cost, or personnel requirements.
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