Fig. 3 | Scientific Reports

Fig. 3

From: A stable combination of non-stable genes outperforms standard reference genes for RT-qPCR data normalization

Fig. 3

Pseudocode of the gene combination method. In this case, k is set to 3 genes, the gene combination method relies on a triple loop aiming at selecting the optimal 3-genes among a set of N genes whose expressions are extracted from an RNA-Seq dataset containing a wide range of conditions that approximate our conditions of interest. The optimal 3-genes is then a 3-genes having a mean expression greater or equal to the target gene mean expression and a minimal variance.

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