Fig. 4 | Scientific Reports

Fig. 4

From: A stable combination of non-stable genes outperforms standard reference genes for RT-qPCR data normalization

Fig. 4

Pipeline for selecting and ranking candidate genes. The pipeline carried out for each target gene is built as follows: in silico selection of candidate genes (boxes a, b, c, d) and in vivo qPCR expression measurements (boxes f, g, h) are followed by in silico and in vivo rankings (boxes e, i). The pseudocode aiming at mining LVkGs (box d) is detailed in Fig. 3. Blue and green boxes correspond respectively to the processes aiming at selecting and ranking candidate genes in silico (from TomExpress database expressions) and in vivo (from qPCR measurements). Comparison of the obtained independent rankings is shown by a grey double arrow.

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