Fig. 3
From: FN1 shapes the behavior of papillary thyroid carcinoma through alternative splicing of EDB region
EDB(−)FN1 inhibits cell proliferation by upregulating p53-targeted protein p21 expression. (A) The Venn plot showing the genes in p53 signaling pathway downregulated after knockdown of FN1 and re-upregulated after overexpression of EDB(−)FN1. (B) qRT-PCR analysis verifying P21, BAX, ZMAT3 and MDM2 expression in indicated cells. (C) Knockdown of alternative splicing EDB domain of FN1 verified by western blotting and semiquantitative PCR with GAPDH loading control in K1 and KTC-1 cells. (D, E) Western blot analysis of P53 and p21 expression level in different transfection cells. (F, G) PTC cells treated with DNA-induced damage drug cis-platinum for 24 h, then Western blotting analyzed P53 and p21 expression in shFN1 and shEDB cells. (H) Cell proliferation ability of shFN1 cell overexpressed EDB(−)FN1 or EDB(+)FN1 isoform measured by CCK8 assays. (I, J) After shEDB and shFN1 cells transduced with sgRNAs targeting p53 and p21, the cell growth capability tested by CCK8 assays.
