Fig. 6 | Scientific Reports

Fig. 6

From: Deficiency of purinergic P2Y2 receptor impairs the recovery after renal ischemia-reperfusion injury and accelerates renal fibrosis and tubular senescence in mice

Fig. 6

JunB deficiency alters cell cycles and attenuates the expression of fibrogenic mediators and cytokines in proximal tubular HK2 cells. Cells were transfected with siRNA of scrambled, P2Y2R, or JunB for 24 h, and treated with TGF-β (5 ng/ml) for 24, 48, or 72 h. (A) After transfection with P2Y2R siRNA, relative P2Y2R mRNA expression was determined by real-time PCR analysis. Relative expression of JunB, p21, cyclin D1, cyclin E, and β-actin was analyzed by western blot and quantified. (B) After transfection with P2Y2R or JunB siRNA, relative JunB mRNA expression was determined by real-time PCR analysis. Percentage of cells in respective cell cycle stages after treatment of scrambled or siRNA of P2Y2R or JunB for 48 h. (C) Relative mRNA expression of TNF-α, MCP-1, MIP-2, and IL-6 was determined by real-time PCR analysis. (D) Relative mRNA expression of fibronectin and collagen I was determined by real-time PCR analysis. Data are presented as mean ± SEM. **P < 0.01, ***P < 0.001 vs. vehicle group of scramble siRNA. #P < 0.05, ##P < 0.01, ###P < 0.001 vs. respective TGFβ treatment group of scramble siRNA.

Back to article page