Fig. 3
From: Lower vicine content reduces the reproductive yield performance in faba bean (Vicia faba L.)
Estimation of vicine from seed samples. (A) Vicine concentration (g/kg) of 189 faba bean accessions based on spectrophotometric estimation, accessions with the highest (Fb-33) and lowest (Fb-40) vicine concentrations are highlighted in red and green respectively. (B) Chromatogram of vicine obtained from the sample Fb-33 in liquid chromatography (retention time- 3.117 min, wavelength-273 nm). (C) Chromatogram of vicine obtained from the sample Fb-40 in liquid chromatography (retention time- 3.117 min, wavelength-273 nm). (D) Mass-spectrum of vicine, which shows a base peak of 305.5 as obtained from LC–MS/MS estimation of vicine from faba bean.
