Fig. 3

HFD-Renin HFpEF results in increased cardiac fibrosis. Representative histology with picrosirius red and fast green counterstain of (A) whole heart (scale bar 3 mm) and (B) 20x magnification (scale bar 200 μm) demonstrating perivascular fibrosis, quantified from two separate regions in n = 4 independent samples. (C) QT-PCR from cardiac tissue for gene markers of fibrosis and myofibroblast activation, n = 6. Data displayed as mean ± SEM. One-way analysis of variance (ANOVA) followed by multiple-comparisons test. P values < 0.05 displayed on graphs.