Fig. 1 | Scientific Reports

Fig. 1

From: Epigenetic drug screening identifies enzyme inhibitors A-196 and TMP-269 as novel regulators of sprouting angiogenesis

Fig. 1The alt text for this image may have been generated using AI.

TeloHAEC bead assay optimization. Representative confocal microscopy images of TeloHAECs with different densities of fibrin gel at day 5. TeloHAECs showing highly proliferative phenotype using 2.5 mg/ml fibrinogen A) with 10 ng/ml VEGF-A stimulation and B) without VEGF-A stimulus. C) 5 mg/ml and 7 mg/ml fibrinogen concentrations reduced the excessive EC proliferation and allowed sprout formation. D) 7 mg/ml fibrinogen concentration inhibited EC sprouting despite VEGF-A stimulus. In all images F-actin is stained using phalloidin-A635 (red), and nuclei with DAPI (cyan). Scale bars, 200 µm. Quantitative analysis of E) sprout length and F) nuclei number. n = 3–5 beads/group were analyzed using SproutAngio tool 16.

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