Fig. 2 | Scientific Reports

Fig. 2

From: Epigenetic drug screening identifies enzyme inhibitors A-196 and TMP-269 as novel regulators of sprouting angiogenesis

Fig. 2The alt text for this image may have been generated using AI.

Endothelial sprouts are formed in TeloHAEC fibrin bead assay in both normoxia and hypoxia. Representative confocal microscopy images of TeloHAEC fibrin bead assay samples at day 5 under A) normoxic and B) hypoxic conditions showing EC sprouting. In all images, F-actin is stained using phalloidin-A635 (red), and nuclei with DAPI (cyan). Scale bar, 200 µm. C-F) Quantitative image analysis showed increased EC sprouting in hypoxic condition. Images were analyzed using SproutAngio tool. n = 12–15 beads/group, 2 independent experiments. G) Human angiogenesis protein array result showed the difference between normoxic and hypoxic conditions in TeloHAEC bead assay (n = 2, 2 independent experiments). VCBM: Vascular cell basal medium.

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