Fig. 6 | Scientific Reports

Fig. 6

From: Epigenetic drug screening identifies enzyme inhibitors A-196 and TMP-269 as novel regulators of sprouting angiogenesis

Fig. 6The alt text for this image may have been generated using AI.

Anti-angiogenic effects of HDAC class IIa inhibitor compound TMP-269. A) Representative confocal microscopy images of DMSO control and TMP-269 (10 µM) treatment groups in HUVEC fibrin bead assay with 10 ng/ml VEGF-A supplement. F-actin is stained using phalloidin-A635 (red), and nuclei with DAPI (cyan). Scale bar, 200 µm. B) Human angiogenesis protein array result showing the difference in the levels of angiogenesis related proteins. C) Quantitation of EC sprouting parameters sprout number, length and width showing the anti-angiogenic effect of TMP-269 treatment. Images were analyzed using SproutAngio tool. n = 3–5 beads/group. D-E) 3’mRNA sequencing enrichment analysis results for fibroblasts (C) and HUVECs (D) treated with TMP-269 compound at 72 h timepoint.

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