Fig. 2
Effect of LRG1 on renal atrophy and renal fibrosis. (a) Experimental workflow for the uIRI models in WT and KO mice. Left kidneys from WT C57BL/6J mice were subjected to 30 min of ischemia. Unoperated right kidneys were used as controls (Sham). (b–d) Relative weight of post-IRI kidneys (I/R) compared to unoperated kidneys (Sham) in WT and KO mice was quantified (n = 6). (e) Representative images of kidneys stained with MTC (original magnification, × 200; scale bar, 100 μm) (n = 6). (f) Representative images of kidneys stained with PSR (original magnification, × 200, bar; scale 100 μm) (n = 6). (g,h) Computerized quantification of the MTC and PSR stained areas (n = 6). (i,j) Relative renal mRNA expression of Col1 and Col3 (n = 6). Values are presented as means ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001 when compared between indicated groups by 1-way ANOVA (c,d) and 2-way ANOVA (b,g,h,i and j) with Bonferroni’s post hoc test. WT wildtype, KO LRG1 knockout, I/R ischemia–reperfusion, uIRI unilateral IRI, MTC Masson’s trichrome, PSR picrosirius-red, Col collagen; ●, WT; ○, KO.
