Fig. 4
Effect of LRG1 on the IRI-induced infiltration of immune cell into the kidney. (a) Representative images of kidneys stained with F4/80 on postoperative day 28 (original magnification, × 400; scale bar, 100 μm). ▲ indicates F4/80 positive cells. (b) The number of F4/80-positive cells in kidney tissue from WT and LRG1 KO mice on postoperative day 28. Five fields were evaluated per mouse (n = 6). (c) Representative images of kidneys stained with Ly6G on postoperative day 28 (original magnification, × 400; scale bar, 100 μm). ▲ indicates Ly6G positive cells. (d) The number of Ly6G-positive cells in kidney tissue from WT and LRG1 KO mice on postoperative day 28. Five fields were evaluated per mouse (n = 6). (e) Representative images of kidneys stained with KIM-1 on postoperative day 28 (original magnification, × 200, bar; scale 100 μm). (f) Computerized quantification of the KIM-1-stained areas (n = 6). Values are presented as means ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001 when compared between indicated groups by 2-way ANOVA with Bonferroni’s post hoc test. WT wildtype, KO knockout, I/R ischemia–reperfusion, KIM-1 kidney injury molecule 1; ●, WT; ○, KO.
