Fig. 3 | Scientific Reports

Fig. 3

From: Puerarin pretreatment provides protection against myocardial ischemia/reperfusion injury via inhibiting excessive autophagy and apoptosis by modulation of HES1

Fig. 3The alternative text for this image may have been generated using AI.

Pue prevents MPTP opening and Lyso-Tracker Red production from injury induced by A/R. (A, B) Flow cytometry BBcellProbe M61 assay for cellular MPTP following Pue, RA or 3MA pre-treatment. (C, D) Fluorescent probe LysoTracker Red for detecting lysosomes and quantitative analysis following treatment (enlargement, 200×; scaled bar: 50 μm). Data are showed as the mean ± SD (n = 3). *P < 0.05, **P < 0.01, ***P < 0.001. Pue, Puerarin; RA, rapamycin; 3MA, 3-methyladenine; A/R, anoxia/reoxygenation; MPTP, mitochondrial permeability transition pore; ns, not significant.

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