Fig. 6 | Scientific Reports

Fig. 6

From: Puerarin pretreatment provides protection against myocardial ischemia/reperfusion injury via inhibiting excessive autophagy and apoptosis by modulation of HES1

Fig. 6

Pue alleviates apoptosis caused by A/R injury by mediating HES1. (A) Western bloting analysis for the detection of protein expression and quantitative analysis of BCL2 and Bax in cell lysates after Pue, pAD/HES1-shRNA or NC treatment. (B) Caspase-3 activity, (C, D) Apoptosis was detected using the Annexin V-FITC/PI via flow cytometry. Data are showed as the mean ± SD (n = 3). *P < 0.05, **P < 0.01, ***P < 0.001. Pue, Puerarin; NC, negative control; A/R, anoxia/reoxygenation; BCL2, B‑cell Lymphoma-2; Bax, BCL2‑associated X protein; ns, not significant.

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