Table 5 Hematological parameters of Nile tilapia (Oreochromis niloticus) fed on normal and high fat diets supplemented with low and high dose of clenbuterol at both level of dietary fat for 8 weeks.

From: Assessing clenbuterol’s modulation of metabolic and inflammatory pathways in Nile tilapia (Oreochromas niloticous) fed high fat diet

Parameters

F6Clen0

F6CLen5

F6Clen10

F12Clen0

F12Clen5

F12Clen10

P value of two-way ANOVA

Fat

Clenbuterol

Interaction

RBCs

(× 106/µL)

2.87 ± 0.03b

3.09 ± 0.05a

3.22 ± 0.1a

2.8 ± 0.03b

2.541 ± 0.3c

3.24 ± 0.06a

0.0217

0.0019

0.1248

Hb (g/dL)

8.71 ± 0.04cc

9.47 ± 0.13b

9.88 ± 0.08a

8.00 ± 0.08d

8.43 ± 0.08cd

9.57 ± 0.1ab

< 0.0001

< 0.0001

0.0013

PCV (%)

28 ± 0.63a

30.33 ± 0.68a

31.33 ± 1.12a

26.67 ± 2.62b

24.66 ± 0.73b

31.66 ± 0.68a

0.0115

0.0014

0.0848

MCV (fL)

97.56 ± 1.02a

98.16 ± 0.65a

97.29 ± 0.41

95.25 ± 1.51b

97.09 ± 3.50a

97.72 ± 7.17a

0.0416

0.0176

0.9329

MCH (pg)

30.35 ± 0.21a

30.65 ± 0.22a

30.68 ± 0.13a

28.57 ± 0.65b

33.19 ± 0.29a

29.54 ± 0.23b

0.0160

< 0.0560

< 0.0360

MCHC (g/dL)

31.11 ± 0.54a

31.22 ± 0.02a

31.54 ± 0.21a

29.9 ± .042b

34.18 ± 0.32a

30.23 ± 0.35a

0.0142

0.0346

0.0244

WBCs (× 103/μL)

20.80 ± 0.41b

22.01 ± 0.68ab

21.36 ± 0.3ab

24.80 ± 1.39a

19.45 ± 0.4b

22.61 ± 1.36ab

0.2245

0.0815

0.0040

Heterophils (× 103/μL)

3.22 ± 0.001a

2.55 ± 0.13ab

2.2 ± 0.06b

3.15 ± 0.22a

2.3 ± 0.22b

2.59 ± 0.31ab

0.8821

0.0003

0.2324

Lymphocytes (× 103/μL)

15.71 ± 0.51b

17.27 ± 0.77ab

17.39 ± 0.79ab

19.32 ± 0.98a

15.08 ± 0.49b

17.91 ± 0.96ab

0.3118

0.1295

0.0039

Monocytes (× 103/μL)

1.46 ± 0.03bc

1.83 ± 0.03ab

1.49 ± 0.08ac

1.90 ± 0.16a

1.35 ± 0.04c

1.74 ± 0.15ab

0.3468

0.6201

0.0002

Eosinophils (× 103/μL)

0.21 ± 0.003

0.22 ± 0.01

0.15 ± 0.02

0.17 ± 0.07

0.23 ± 0.02

0.23 ± 0.004

0.4973

0.3539

0.1444

Basophils (× 103/μL)

0.20 ± 0.13

0.15 ± 0.01

0.14 ± 0.01

0.25 ± 0.01

0.22 ± 0.01

0.14 ± 0.01

0.3683

0.3001

0.7571

  1. Hematological parameters of Nile tilapia (Oreochromis niloticus) fed on normal and high fat diets supplemented with low and high dose of clenbuterol at both level of dietary fat for 8 weeks. RBCs; red blood corpuscles, Hb; Hemoglobin, PCV; packed cell volume, MCV; Mean Cell Volume, MCH; Mean Cell Hemoglobin. WBCs; White blood cells. Data are expressed as Mean ± SEM where n = 5. For each parameter, values with different superscripts are significantly different (p < 0.05). Small letters indicate significant differences (two-way ANOVA).