Fig. 1 | Scientific Reports

Fig. 1

From: Antitumor immunostimulatory effect via cell-killing action of a novel extracorporeal blood circulating photodynamic therapy system using 5-aminolevulinic acid

Fig. 1

Antitumor immunostimulatory effect of intravenous administration of 5-ALA/PDT-treated cells. (a–c) Tumor volume changes in individual rats after allogenic C6 cell transplantation. Prior to transplantation, intravenous administration of (a) PBS (control), (b) 1 mmol/L 5-ALA/PDT-treated C6 cells with low irradiation (7.5 J/cm2), or (c) 1 mmol/L 5-ALA/PDT-treated C6 cells with high irradiation (317 J/cm2), was performed three times (day -21, -14, and -1). **p < 0.01 compared with the control group. (d) Photos of the tumors of three rats from each group taken on day 10. (e) Immunostaining of CD8+ cells in the tumors in each group. (f) The average number of CD8+ cells per field (400 ×). (g) IFN-γ level (pg/mL) in supernatants of splenocytes stimulated with C6 cells for 24 h on day 10 was detected by ELISA. Values are presented as mean (SD) **p < 0.01 compared with the control group.

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