Fig. 4 | Scientific Reports

Fig. 4

From: Nidogen-1 suppresses cell proliferation, migration, and glycolysis via integrin β1-mediated HIF-1α downregulation in triple-negative breast cancer

Fig. 4

NID1 downregulated HIF-1α and its target gene expression. (A) Western blot analysis of total cell lysates with the indicated antibodies; GAPDH was used as the loading control. (B) RT-qPCR analysis showing the mRNA expression of HIF1A in the indicated cell lines. Values were normalized to GAPDH. (C) Dual luciferase assays were performed using HRE (hypoxia response element)-luc constructs. pRL-CMV was used as the internal control. (D) RT-qPCR analysis showing the mRNA expression of HIF-1α target genes in the indicated cell lines. Values were normalized to GAPDH. (E) Dual luciferase assays were performed using HRE-luc constructs. pRL-CMV was used as the internal control. Each assay was performed after 24 h of treatment with rhNID1 (100 ng/ml. Data in (B, C, D, and E) are presented as mean ± SD. P values in (B, C, D, and E) were calculated using Student’s t test (*P < 0.05; **P < 0.005; ***P < 0.0005).

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