Fig. 2

Histochemical staining of colon tissue section. (A) Immunohistochemical staining for macrophage marker protein F4/80. (B) Statistical analysis of relative positive area based on the staining in panel. (C) AB-PAS staining, where acidic mucus appears light blue, and glycogen and neutral mucus appear violet. (D) Statistical analysis of mucus granule size based on the staining in panel. (E) Scale bars are indicated in the figures. E. Immunofluorescence staining for the tight junction protein ZO-1.