Fig. 5
LEfSE (LDA Effect Size), Bugbase and PICRUSt 2 analyses. (A) The LDA score and enriched taxa of the intestinal microbiota after neonatal hypoxia, compared with normoxia. The colors of the bars represent different groups, while the lengths of the bars indicate the LDA scores, reflecting the degree of impact of significantly different species between different groups. (B) The enriched taxa in the control and neonatal hypoxia microbiota are represented through a cladogram. In the evolutionary tree plot, the circles radiating from the inside out represent taxonomic levels from phylum to genus. Each small circle at different taxonomic levels represents a classification at that level, and the diameter of the small circle is proportional to its relative abundance. Coloring principle: Species without significant differences are uniformly colored yellow, while Biomarker species with differences are colored according to their respective groups. Red nodes indicate microbial taxa that play an important role in the red group, and green nodes indicate microbial taxa that play an important role in the green group. The species names represented by English letters are displayed in the legend on the right (for aesthetic purposes, only the different species from phylum to family are displayed by default on the right). Note: The species names represented by English letters are also displayed in the legend at the bottom of the figure. (C) Phenotypic characteristic analysis of Bugbase tool’s prediction. The three lines in the graph, from top to bottom, represent the upper quartile, mean, and lower quartile, respectively. (D) The predicted differences in the KEGG metabolic pathways (Shown top 10). (E) The predicted differences in the GBM (Gut-Brain) metabolic pathways (Shown top 10).
