Fig. 6

GDF15 attenuates ferroptosis induced by H/R injury by upregulating GPX4. (A-B) Changes of GDF15 protein expression before and after transfection. (C-D) The levels of cell viability and LDH release. (E-F) Analysis of TUNEL staining results. (D-F) The protein expression of GDF15 in 3 groups. (G-I) The concentration of MDA, SOD and GSH in cell. (J-L) Western blots bands and histograms of ACSL4 and SLC7A11. (M) Predictive structure of GDF15 and GPX4 protein interactions. Data are shown as means ± SEM, *p < 0.05 and **p < 0.01.