Fig. 7
Effect of EMC on the levels of phosphorylated c-Myc at Ser-62 in EATCs. EATCs were treated with or without 100 µM EMC for 6 h. The levels of (A) c-Myc and (B) phosphorylated c-Myc at Ser-62 were determined by Western blot analysis. The images of c-Myc and GAPDH were obtained from the same blot with different exposure times. After detecting c-Myc, the membrane was stripped of the c-Myc antibody and reprobed with GAPDH antibody to obtain the GAPDH bands. The images of the phosphorylated c-Myc at Ser-62 and GAPDH were obtained from the same blot but with different exposure times. (C) Results show the ratio of phosphorylated c-Myc at Ser-62 to c-Myc. Results are expressed as mean ± SD (n = 4). Statistical significance between the two groups was evaluated using Student’s t-test. The data were found to be significantly different comparison to the control: ** p < 0.01.
