Table 1 Primer sequences.

From: Ethyl p-methoxycinnamate inhibits tumor growth by suppressing of fatty acid synthesis and depleting ATP

Gene

Primer sequences (5’ → 3’)

Tm (℃)

GeneBank No.

Acly

CCTTTGACAGCGGCATCATT

63.4

NM_001199296.1

 

TTCAGGATAAGATTTGGCTTCTTGG

63.8

 

Acc1

CTGCAGAAACTCATCCTCTCGG

63.6

NM_133360.3

 

GCGGTGTTGTACGCTGTTGA

63.2

 

Fasn

GAATACACAGCCACCGACCG

63.8

NM_007988.3

 

CACCAGAAGGTCAAGGGCAC

63.0

 

Cpt1a

GGCTATGGTCAAGGTCTTCTCG

62.4

NM_013495.2

 

GTGCTGTCATGCGTTGGAAGT

63.6

 

Cpt1b

CAGGCAAAGAGACAGACTTGCTA

61.1

NM_009948.2

 

GCCCTCATAGAGCCAGACCTT

62.0

 

SREBP1

GAGCTGCGTGGTTTCCAACA

64.6

NM_011480.4

 

GTGGCCTCATGTAGGAATACCCTC

64.1

 

b-actin

CATCCGTAAAGACCTCTATGCCAAC

64.1

NM_007393.5

 

ATGGAGCCACCGATCCACA

64.9

 
  1. Acly: ATP citrate lyase, Acc1: acetyl-CoA carboxylase alpha, Fasn: fatty acid synthase, Cpt1a: carnitine palmitoyltransferase 1a, Cpt1b: carnitine palmitoyltransferase 1b, SREBP1: sterol regulatory element binding transcription factor 1, and β-Actin: Beta-actin.
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