Fig. 3
From: A novel cardiomyopathy phenotype linked to a CHD7 missense variant
Comparative analysis and quantitation of cardiomyopathy in E18.5 mouse embryos. (a) Cardiac imaging and 3D reconstruction. Cross-sectional views of hearts are shown on the left, with 3D reconstructions highlighting the left ventricular wall (yellow) and right ventricular chamber (green) in the middle panel. The right panel shows the ventricular wall and septum. Three phenotypes are shown: normal, enlarged ventricular chamber (DCM), and reduced ventricular chamber (HCM). (b-c) Quantitative analysis of cardiac morphology. Ratios of the volumes of the left and right ventricular chamber were normalized to total ventricle volumes by measuring individual volumes in cubic millimeters (mm³). Comparisons were made among Chd7+/+ (WT), Chd7fs/+, Chd7T720A/+, Chd7T720A/T720A, and Chd7fs/T720A mice. Z scores were calculated using the average and standard deviation of WT, with significant thresholds marked at Z = ± 2. DCM and HCM phenotypes are color-coded in pink and blue, respectively.
