Fig. 4
NMDAR Antagonists Mitigate Myelination Deficits Induced by COVID-19 Infection. (a) Visualization of myelination in samples of medulla among key groups using black-gold II staining. Key groups include those sacrificed 10 days after COVID-19 inoculation, with representative images of samples either positive or negative for COVID-19 and treated with saline (control), memantine, or ifenprodil. Representative images for all other groups can be found in Supplementary Fig. 2. Each image is shown in the 4 × and 10 × magnification with anatomical markers labeled. (b) Examples of images representative of each semi-qualitative rank for Black-Gold II stain. (c) Representative Nissl (left) and anatomical annotations (right) from the Allen Brain Reference Atlases—Adult Mouse Brain showing i) pons and ii) medulla for reference119,120,121,122,123,124. (d) Black-Gold II Myelin semi-qualitative rankings according to time sacrificed post-virus inoculation and treatment group. Individual data points are representative of mode ranking values from sections in pons and medulla. Error bars represent mode qualitative ranking + /− S.E.M. Significant differences between groups are denoted with **p < 0.01. N = 5–18 sections per group.
